Anaerobic incubator case: bifidobacterium culture

 Anaerobic incubator case: bifidobacterium culture


The culture medium formula of bifidobacterium can refer to the national standard of Microbiology test Methods for food hygiene GB/T4789-2003, which is the latest version in 2003, and the culture and identification of bifidobacterium and lactic acid bacteria are newly added. I have made a culture medium with reference to the NATIONAL standard, but it is not BBL. I am sorry that I can not remember the name at that time. The ingredients in the formula are simpler than BBL, and there is no need to do a tomato extraction solution.


In addition, the culture needs at least 72 hours, if the experimental conditions are simple, it is best to use pyrogallic acid and anhydrous sodium carbonate reaction method is better, simple operation, good effect. Focal gallic acid and anhydrous sodium carbonate 2 grams each said (says good in advance, standby), to prepare than to use a dish of AGAR (say good medicine can be put on the above, do not mix), and ready to the melted wax, after being culture pour into AGAR, waiting for solidification, mix drugs, then buckle the solidification of AGAR in drug larger AGAR plates, Be careful not to make the medium plane contact with the drug, and seal the contact between the two plates with wax to form an anaerobic environment inside, and count after 72 hours. After 48 hours of culture, the growth is not very good, the colony is a little small, not full.

Anaerobic incubator


评论

发表评论

此博客中的热门博文

What incubator should I use for fungi?

图片
There are many different types of incubators and the choice of the incubator should depend on what is being cultured. If the microorganism to be cultured is aerobic, then a normal incubator is sufficient; if it is anaerobic, then you need to buy an anaerobic incubator; if it is algae or fungi, it is better to have a light incubator. A light incubator is a piece of high-precision constant temperature equipment with light function; a light incubator is a special constant temperature incubation device for the cultivation of bacteria, molds, microorganisms, and breeding tests, especially practical for biological engineering, medical research, agriculture, and forestry science, aquaculture, animal husbandry and other fields engaged in scientific research and production use of the ideal equipment.　 The main technical parameters of the fungal incubator in the microbiology room of the laboratory require 1: Temperature requirements: 2 ~ 48 c, the temperature inside the incubator temperature u
阅读全文

Laminar flow Cabinet architecture introduction

图片
Laminar Flow Cabinet Clean Bench, also known as laminar flow Cabinet, can be mainly divided into two- person single, single side double side, horizontal vertical, etc. The structure is divided into the box, table, bracket, support foot, exhaust pipe, HEPA filter, pre-filter, etc.   Laminar flow cabinet design should have sufficient strength and stiffness, the weight should be light under the premise of good stability, the joints should be tight, according to the needs of the support foot can be self-locking structure universal wheel, but for the high requirements of seismic workbench without support feet and universal wheel, the operating area of the working surface can be made independent of the box platform. The inner surface of the single table is made of three sides of the wall. The wall surface is smooth and the joint is sealed. The glass and metal connection parts should also be sealed. The negative pressure air duct should be designed so that the air duct, plenum, filter, and su
阅读全文

The Control system is the core of the constant temperature and humidity chamber

图片
The Control system is the core of the constant temperature and humidity chamber , it determines the temperature rise rate of the text chamber, precision, and other important indicators. Now the controller of the test chamber mostly adopts PID control, but also a few adopts PID and fuzzy control phase combination control mode. Because the control system of the thermostatic oscillator basically belongs to the category of software, and this part in the use of the process, generally there will be no problems. In the constant temperature and humidity chamber refrigeration principle diagram, we introduced the temperature and humidity chamber refrigeration system several important components of the working path. So how do these components work? The refrigerant is adiabatically compressed to a higher pressure by the compressor, and the work is consumed to raise the exhaust temperature. After that, the refrigerant isothermally exchanges heat with the surrounding medium through the refrigerant c
阅读全文