Biochemical incubator application: compost extract seed germination rate determination

Biochemical incubators are most common in laboratories. Biochemical incubators are widely used, such as seed germination rate determination of compost extract.

The extract of the compost sample was extracted, and the germination rate of seeds was measured. According to the literature, a piece of filter paper was spread in a 9cm petri dish, 20 seeds (radish seeds were used) were placed, and 5ml extract was added (I added 10ml). The seeds were cultured in a biochemical incubator at 25 oC for 5 days.

Calculate the formula to calculate the speed germination index can be. The ions were removed as a blank control.


Calculation formula: F= (A1×A2) /(B1×B2)×100%


F: seed germination index;


A1: germination rate of seeds cultured infiltrate of compost samples;


A2: Root length of seeds cultured infiltrate of compost sample;


B1: germination rate of seeds cultured in deionized water;


B2: Root length of seeds cultured in deionized water.

评论

发表评论

此博客中的热门博文

What incubator should I use for fungi?

图片
There are many different types of incubators and the choice of the incubator should depend on what is being cultured. If the microorganism to be cultured is aerobic, then a normal incubator is sufficient; if it is anaerobic, then you need to buy an anaerobic incubator; if it is algae or fungi, it is better to have a light incubator. A light incubator is a piece of high-precision constant temperature equipment with light function; a light incubator is a special constant temperature incubation device for the cultivation of bacteria, molds, microorganisms, and breeding tests, especially practical for biological engineering, medical research, agriculture, and forestry science, aquaculture, animal husbandry and other fields engaged in scientific research and production use of the ideal equipment.　 The main technical parameters of the fungal incubator in the microbiology room of the laboratory require 1: Temperature requirements: 2 ~ 48 c, the temperature inside the incubator temperature u
阅读全文

Laminar flow Cabinet architecture introduction

图片
Laminar Flow Cabinet Clean Bench, also known as laminar flow Cabinet, can be mainly divided into two- person single, single side double side, horizontal vertical, etc. The structure is divided into the box, table, bracket, support foot, exhaust pipe, HEPA filter, pre-filter, etc.   Laminar flow cabinet design should have sufficient strength and stiffness, the weight should be light under the premise of good stability, the joints should be tight, according to the needs of the support foot can be self-locking structure universal wheel, but for the high requirements of seismic workbench without support feet and universal wheel, the operating area of the working surface can be made independent of the box platform. The inner surface of the single table is made of three sides of the wall. The wall surface is smooth and the joint is sealed. The glass and metal connection parts should also be sealed. The negative pressure air duct should be designed so that the air duct, plenum, filter, and su
阅读全文

The Control system is the core of the constant temperature and humidity chamber

图片
The Control system is the core of the constant temperature and humidity chamber , it determines the temperature rise rate of the text chamber, precision, and other important indicators. Now the controller of the test chamber mostly adopts PID control, but also a few adopts PID and fuzzy control phase combination control mode. Because the control system of the thermostatic oscillator basically belongs to the category of software, and this part in the use of the process, generally there will be no problems. In the constant temperature and humidity chamber refrigeration principle diagram, we introduced the temperature and humidity chamber refrigeration system several important components of the working path. So how do these components work? The refrigerant is adiabatically compressed to a higher pressure by the compressor, and the work is consumed to raise the exhaust temperature. After that, the refrigerant isothermally exchanges heat with the surrounding medium through the refrigerant c
阅读全文