How to use anaerobic incubator for cell anaerobic experiment?

Do cell anaerobic experiment, need cell anaerobic culture to do a follow-up, had better use to the anaerobic incubator.



Process: After the medium is prepared, it is separately packed in the screw mouth test tube, and the screw cap is released for high-pressure sterilization at 121 ℃ for 20 minutes. After sterilization, it is quickly put into the sampling chamber of the anaerobic incubator which has been inflated and replaced for one hour. After vacuuming and filling with nitrogen, the medium is taken into the operation room by opening the inner door (the main purpose is that the sterilization process of the medium is also dissolved out of it).

Screw the screw cap tightly, and then carry out the corresponding dilution and mixing operation in the operating room. Then screw the lid tightly, take out the anaerobic tank, seal with sealing glue, and roll the test tube, and then culture at 55 ℃.

Control table operation of the anaerobic incubator,

Click the "set" key to enter into the temperature setting state. The prompt "SP" is displayed on the upper row of the display screen, and the temperature setting value (the first-bit value flickers) is displayed in the lower row of the display screen, which can be modified to the required setting value through the shift, increase and decrease keys;

Then click the "set" key to exit the setting state and the modified set value will be saved automatically; then click the "set" key to enter into the constant temperature time setting state, and the prompt "ST" will be displayed in the upper row of the display screen, and the set value of the constant temperature time will be displayed in the lower row (the first digit value will flash); Then click the "set" key to exit the setting state, and the modified setting value will be saved automatically.

When the constant temperature time is set to "0", there is no timing function.

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